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CD45RO Mouse anti-Human, Brilliant Violet 605, Clone: UCHL1, BD

Mouse Monoclonal Antibody

$201.00 - $406.00

Specifications

Antigen CD45RO
Isotype IgG2a, κ
Immunogen Human IL-2-dependent T-cell line
Formulation Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Conjugate Brilliant Violet 605
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Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
BDB562790
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BD Biosciences
562790
25 Tests
Each for $201.00
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BDB562791
View Documents Promotion Details
BD Biosciences
562791
100 Tests
Each for $406.00
Add to cart
 
Description

Description

The UCHL1 monoclonal antibody specifically binds to the 180 kDa isoform of CD45 (aka, the Leukocyte Common Antigen). CD45RO is a type I transmembrane glycoprotein that has cytoplasmic protein tyrosine phosphatase activity and functions in signal transduction pathways. This CD45 isoform does not include amino acid sequences encoded by the variable CD45 exons A, B, or C. CD45RO is expressed on most thymocytes, activated T cells, memory T cells, granulocytes and monocytes, but only on a proportion of resting T cells. CD45RO and CD45RA antibodies seem to define complementary, predominantly non-overlapping, populations in resting peripheral T cells, demonstrating heterogeneity within the CD8 and CD4 subpopulations. CD45RO binds to CD22.

This antibody is conjugated to BD Horizon BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.

Specifications

Specifications

CD45RO
Human IL-2-dependent T-cell line
Brilliant Violet 605
Flow Cytometry
RUO
UCHL1
CD45R; PTPRC; LCA; Leukocyte common antigen; GP180; LY5; T200
IgG2a, κ
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Primary
Mouse
Affinity Purified
Monoclonal
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
SDS
Product Certifications

For Research Use Only.

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