CD45RO Mouse anti-Human, Brilliant Violet 605, Clone: UCHL1, BD

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
BDB562790	BD Biosciences 562790	25 Tests
BDB562791	BD Biosciences 562791	100 Tests

Description

The UCHL1 monoclonal antibody specifically binds to the 180 kDa isoform of CD45 (aka, the Leukocyte Common Antigen). CD45RO is a type I transmembrane glycoprotein that has cytoplasmic protein tyrosine phosphatase activity and functions in signal transduction pathways. This CD45 isoform does not include amino acid sequences encoded by the variable CD45 exons A, B, or C. CD45RO is expressed on most thymocytes, activated T cells, memory T cells, granulocytes and monocytes, but only on a proportion of resting T cells. CD45RO and CD45RA antibodies seem to define complementary, predominantly non-overlapping, populations in resting peripheral T cells, demonstrating heterogeneity within the CD8 and CD4 subpopulations. CD45RO binds to CD22.

This antibody is conjugated to BD Horizon BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.

Specifications

• Antigen: CD45RO
• Applications: Flow Cytometry
• Conjugate: Brilliant Violet 605
• Regulatory Status: RUO
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Isotype: IgG2a κ
• Purification Method: Affinity Purified
• Description: CD45R; PTPRC; LCA; Leukocyte common antigen; GP180; LY5; T200

• Clone: UCHL1
• Classification: Monoclonal
• Host Species: Mouse
• Target Species: Human
• Immunogen: Human IL-2-dependent T-cell line
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

For Research Use Only.