CD49d Rat anti-Mouse, NA/LE, Unlabeled, Clone: R1-2, BD

Rat Monoclonal Antibody

Supplier:  BD Biosciences 553153

Catalog No. BDB553153

Description

The R1-2 antibody reacts with the integrin α;4 chain (CD49d), which is expressed as a heterdimer with either of two β;, β;1 or β;7 (also known as β;p). The α;4β;1 integrin (VLA-4, CD49d/CD29) is expressed on most peripheral lymphocytes, thymocytes, and monocytes; while the α;4β;7 integrin (LPAM-1) is expressed on peripheral lymphocytes, but on only a small subset of thymocytes. These integrins mediate a variety of cell-cell and cell-matrix interactions, recognizing the ligands VCAM-1 (CD106) and fibronectin. There is evidence that levels of VLA-4 expression regulate the transendothelial migration of T lymphocytes into inflamed tissues. Integrin α;4β;7 also preferentially binds to the mucosal vascular addressin, MAdCAM-1. The R1-2 antibody blocks some α;4 integrin-mediated binding functions. In combination with mAb 9C10 (MFR4.B) (Cat. No. 553313), binding of VLA-4 expressing cells to VCAM-1 can be almost completely inhibited.

Specifications

• Antigen: CD49d
• Classification: Monoclonal
• Concentration: 1.0mg/mL
• Description: Integrin α4 chain
• Host Species: Rat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C

• Applications: Flow Cytometry
• Clone: R1-2
• Conjugate: Unconjugated
• Formulation: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered. Endotoxin level is ≤0.01 EU/μg (≤0.001 ng/μg) of protein as determined by the LAL assay.
• Immunogen: AKR/Cum mouse spontaneous T lymphoma line TK1
• Quantity: 0.5 mg
• Research Discipline: Immunology
• Target Species: Mouse
• Isotype: IgG2b κ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only.