CD4a Mouse anti-Pig, Alexa Fluor 647, Clone: 74-12-4, BD

Catalog No.	Quantity
BDB561472	50 μg

Catalog No. BDB561472 Supplier BD Biosciences Supplier No. 561472

Description

Mouse Monoclonal Antibody

The 74-12-4 (also known as clone PT4) monoclonal antibody specifically binds to CD4, a 55-kDa antigen expressed on T lymphocytes. This antibody does not react with CTL effectors, CTL precursors, or NK cells (ie, CD8[bright] cells) and it does not cross-react with human or bovine cells. Two peripheral T-helper lymphocyte phenotypes can be distinguished in the pig: CD4+CD8- and CD4+CD8[dull]. mAb 74-12-4 has been reported to inhibit proliferative responses of peripheral blood lymphocytes to mitogen, soluble antigen, and alloantigen. It is only marginally effective for in vivo depletion of peripheral CD4+ T cells. Two alloantigenic forms of CD4 have been recognized in miniature swine based upon their recognition (CD4.1) or lack of recognition (CD4.2) by mAb 74-12-4; the CD4.2 phenotype displays an autosomal recessive, non-MHC-linked, pattern of inheritance. The molecular basis for the polymorphism is a cluster ofnucleotide differences leading to multiple amino-acid substitutions in the Ig CDR2-like loop structure. This mAb was clustered as anti-CD4a at the First International Swine CD Workshop. It has been reported to crossreact with chicken leukocytes.

Specifications

• Antigen: CD4a
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: 74-12-4
• Concentration: 0.2mg/mL
• Conjugate: Alexa Fluor 647
• Description: CD4; CD4 molecule; Lymphocyte antigen CD4
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Mouse
• Immunogen: dd miniature swine thymocytes
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Pig
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG2b κ