CD54 Mouse anti-Rat, PE, Clone: 1A29, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 554970

Catalog No. BDB554970

Description

The 1A29 antibody reacts with ICAM-1 (CD54), an 85 kDa surface glycoprotein expressed on vascular endothelium in lymphoid tissues, thymic stromal cells, peripheral blood monocytes, peritoneal macrophages and mast cells, dendritic cells, and weakly on peripheral lymphocytes and thymocytes. ICAM-1 is a ligand for LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). Its expression is upregulated on activated lymphocytes and endothelial cells. 1A29 mAb inhibits PMA-induced aggregation of PHA-stimulated splenic blasts, as well as the adhesion of mitogen-stimulated blasts to HEV-derived endothelial cells and purified ICAM-1. It has also been reported that 1A29 antibody can inhibit leukocyte infiltration in in vivo systems, blocks induction of experimental allergic encephalomyelitis, and reduces NK-cell adhesion to tumor cells. The effects of in vivo administration of the 1A29 monoclonal antibody have been thoroughly investigated.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Specifications

• Antigen: CD54
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Description: ICAM-1
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: 1A29
• Conjugate: PE
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Rat HEV-Derived Cell Line Ax
• Quantity: 0.2 mg
• Research Discipline: Immunology
• Target Species: Rat
• Isotype: IgG1 κ

For Research Use Only.