CD54 Hamster anti-Mouse, PE, Clone: 3E2, BD

Hamster Monoclonal Antibody

Supplier:  BD Biosciences 553253

Catalog No. BDB553253

Description

The 3E2 antibody reacts with CD54 (ICAM-1), a 95-kDa member of the Ig superfamily found on lymphocytes, vascular endothelium, high endothelial venules, epithelial cells, macrophages, and dendritic cells. ICAM-1 is a ligand for LFA1 (CD11a/CD18) and Mac-1 (CD11b/CD18). Its expression is upregulated upon stimulation by inflammatory mediators such as cytokines and LPS. Studies with mouse Icam1 -transfected antigen-presenting cells, with CD54-blocking antibodies, and in CD54-deficient mice indicate that CD54 participates in inflammatory reactions and antigen-specific immune responses. In addition, there is evidence that CD54 is a receptor involved in MHC-non-restricted responses to weakly immunogenic tumor cells. The 3E2 antibody has been reported to block in vitro and in vivo intracellular adhesion events involved in immune responses.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Specifications

• Antigen: CD54
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Description: ICAM-1; Icam1; Intercellular adhesion molecule 1; Ly-47; MALA-2; MyD10
• Host Species: Hamster
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: 3.00E+02
• Conjugate: PE
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Not reported
• Quantity: 0.2 mg
• Research Discipline: Immunology
• Target Species: Mouse
• Isotype: IgG1 κ

For Research Use Only.