Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Antibodies
  4. Primary Antibodies
  5. All Primary Antibodies
  6. BDB557775

CD56 Mouse anti-Human, BD IMag-DM, Clone: NCAM16.2, BD

Catalog No. BDB557775 Shop All BD Biosciences Products
Change view
Click to view available options
Quantity:
1 x 109 Cells
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
BDB557775 1 x 109 Cells
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. BDB557775 Supplier BD Biosciences Supplier No. 557775
Add to Cart
Add to Cart

Description

Mouse Monoclonal Antibody

BD IMag™ Anti-Human CD56 Magnetic Particles - DM are magnetic nanoparticles that have monoclonal antibody conjugated to their surfaces. These particles are optimized for the positive selection or depletion of the CD56-bearing leukocytes using the BD™ IMagnet. CD56, also known as the neural cell adhesion molecule (N-CAM), is expressed in many different cell and tissue types. In normal donors, it is found on NK and NK-T cells. The NCAM16.2 mAb has been reported to cross-react with lymphocytes of the rhesus macaque. BD IMag™ Anti-Human CD56 magnetic particles have been reported to effectively separate CD56-bearing cells of rhesus macaque blood. For enrichment of NK cells without NK-T cells, the use of BD™ IMag Anti-Human CD3 Magnetic Particles - DM (Cat. no. 552593) are suggested for depleting T lymphocytes before separation of CD56-positive leukocytes.

Peripheral Blood Mononuclear Cells (PBMC) are labeled with BD IMag™ Anti-Human CD56 Magnetic Particles - DM according to the Magnetic Labeling Protocol. This labeled cell suspension is then placed within the magnetic field of the BD IMagnet™. Labeled cells migrate toward the magnet (positive fraction), leaving the unlabeled cells in suspension so they can be drawn off (negative fraction). The tube is then removed from the magnetic field for resuspension of the positive fraction. The separation is repeated twice to increase the purity of the positive fraction. The magnetic separation steps are diagrammed in the Separation Flow Chart. After the positive fraction is washed, the small size of the magnetic particles allows the positive fraction to be further evaluated in downstream applications, such as by flow cytometry.

TRUSTED_SUSTAINABILITY

Specifications

Antigen CD56
Applications Flow Cytometry
Classification Monoclonal
Clone NCAM16.2
Conjugate BD IMag-DM
Description NCAM1; NCAM-1; NCAM; Leu-19; Neural cell adhesion molecule 1; NKH1; MSK39
Formulation Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Host Species Mouse
Immunogen Immunoaffinity-enriched adult human brain NCAM
Quantity 1 x 109 Cells
Regulatory Status RUO
Research Discipline Immunology
Primary or Secondary Primary
Target Species Human
Content And Storage Store undiluted at 4°C
Form Liquid
Isotype IgG2b κ
Show More Show Less

For Research Use Only.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.