CD62L Rat anti-Mouse, Brilliant Violet 605, Clone: MEL-14, BD

Catalog No.	Quantity
BDB563252	50 μg

Catalog No. BDB563252 Supplier BD Biosciences Supplier No. 563252

Description

Rat Monoclonal Antibody

The MEL-14 monoclonal antibody specifically binds to CD62L (L-selectin), a 95 kDa (on neutrophils) or 74 kDa (on lymphocytes) receptor with lectin-like and Epidermal Growth Factor-like domains. In the mouse, L-selectin is detected on most thymocytes, with the highest levels of expression on an immunocompetent subset and a population of dividing progenitor cells, and on peripheral leukocytes, including subsets of B and T lymphocytes, neutrophils, monocytes, and eosinophils. This member of the selectin adhesion molecule family appears to be required for lymphocyte homing to peripheral lymph nodes and to contribute to neutrophil emigration at inflammatory sites. L-selectin is rapidly shed from lymphocytes and neutrophils upon cellular activation; metalloproteinases may mediate the release of CD62L ectodomains from the cell surface. The level of CD62L expression, along with other markers, distinguishes naive, effector, and memory T cells. L-selectin binds to sialytaed oligosaccharide determinants on high endothelial venules (HEV) in peripheral lymph nodes. In vitro studies have demonstrated that CD34, GlyCAM-1, and MAdCAM-1, all recognized by mAb MECA-79 (anti-mouse PNAd Carbohydrate Epitope, Cat. No. 553863), may be ligands for CD62L. MEL-14 mAb blocks in vitro binding of lymphocytes to peripheral lymph node HEV and inhibits in vivo lymphocyte extravasation into peripheral lymph nodes and late stages of leukocyte rolling.

This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon™ BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon™ BV605 is a tandem fluorochrome of BD Horizon™ BV421 and Cy™3.5. Due to the excitation of Cy3.5 by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon™ PE-CF594 detectors off the green or yellow-green lasers. BD Horizon™ BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.

Specifications

• Antigen: CD62L
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: MEL-14
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Violet 605
• Description: Sell; L-selectin; LECAM-1; LAM-1; Lnhr; Ly-22; Ly-m22; Lyam-1
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Host Species: Rat
• Immunogen: C3H/eb mouse B lymphoma 38C-13
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Mouse
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG2a κ