CD64 a and b Alloantigens Mouse anti-Mouse, Alexa Fluor 647, Clone: X54-5/7.1, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 558539

Catalog No. BDB558539

Description

The monoclonal allo antibody X54-5/7.1 reacts with FcγRI (CD64) encoded by the more common Fcgr1a and Fcgr1b alleles. The alloantigens generated by the Fcgr1a and Fcgr1b alleles, have been confirmed positive in mouse strains BALB/c and C57BL/6 and reported positive in strains 129, A, AKR, ALR, BUB, C3H, C57BL/10, C57BLKS, C57BR, C58, CBA, CE, DBA/2, HRS, MRL, NON, NZB, NZO, NZW, PL, SJL, ST, SWR. The a and b alloantigens have been reported negative in mouse strains ABH, NOD. CD64, a key receptor in the development of immune responses, has a dual role as a low affinity receptor for IgG3 and high affinity receptor for IgG2a linking innate and adaptive immunities. CD64 mediates endocytosis, phagocytosis, antibody-dependent cellular toxicity, cytokine release and superoxide generation. CD64 is expressed largely on macrophages and dendritic cells. For more information regarding clone X54-5/7.1 and the alloantigens it recognizes, please refer to the reference by Tan et al listed below.

Specifications

• Antigen: CD64 a and b Alloantigens
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Description: FcgRI
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: X54-5/7.1
• Conjugate: Alexa Fluor 647
• Formulation: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
• Immunogen: Mouse CD64 a Alloantigen
• Quantity: 0.1 mg
• Research Discipline: Immunology
• Target Species: Mouse
• Isotype: IgG1 κ