Learn More
CD69 Hamster anti-Mouse, Brilliant Violet 786, Clone: H1.2F3, BD
Hamster Monoclonal Antibody
Supplier: BD Biosciences 564683
Description
The H1.2F3 monoclonal antibody specifically binds to CD69 (Very Early Activation antigen), an 85 kDa disulfide-linked homodimer of differentially glycosylated subunits. CD69 is a C-type lectin, most closely related to the NKR-P1 and Ly-49 NK cell-activation molecules. Its expression is rapidly induced upon activation of lymphocytes (T, B, NK, and NK-T cells), neutrophils, and macrophages. CD69 is expressed also on thymocytes that are undergoing positive selection; its role in that process is unclear. H1.2F3 mAb augments PMA-induced T-cell stimulation and IFN- γ-induced macrophage stimulation. IL-2-activated NK cells express CD69, and H1.2F3 mAb induces redirected lysis of FcR-bearing target cells by NK cells.
The antibody was conjugated to BD Horizon BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 786-nm. BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60-nm filter).

Specifications
CD69 | |
Monoclonal | |
0.2mg/mL | |
VEA; Very Early Activation Antigen; AIM; Activation Induced Molecule | |
Hamster | |
Affinity Purified | |
RUO | |
Mouse | |
IgG1 λ3 |
Flow Cytometry | |
H1.2F3 | |
Brilliant Violet 786 | |
Aqueous buffered solution containing BSA and ≤0.09% sodium azide. | |
Mouse Dendritic Epidermal T Cell Line Y245 | |
50 μg | |
Primary | |
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
Safety and Handling
Your input is important to us. Please complete this form to provide feedback related to the content on this product.