CD69 Hamster anti-Mouse, Brilliant Violet 510, Clone: H1.2F3, BD

Catalog No.	Quantity
BDB563030	50 μg

Catalog No. BDB563030 Supplier BD Biosciences Supplier No. 563030

Description

Hamster Monoclonal Antibody

The H1.2F3 monoclonal antibody specifically binds to CD69 (Very Early Activation antigen), an 85 kDa disulfide-linked homodimer of differentially glycosylated subunits. CD69 is a C-type lectin, most closely related to the NKR-P1 and Ly-49 NK cell-activation molecules. Its expression is rapidly induced upon activation of lymphocytes (T, B, NK, and NK-T cells), neutrophils, and macrophages. CD69 is expressed also on thymocytes that are undergoing positive selection; its role in that process is unclear. H1.2F3 mAb augments PMA-induced T-cell stimulation and IFN-γ-induced macrophage stimulation. IL-2-activated NK cells express CD69, and H1.2F3 mAb induces redirected lysis of FcR-bearing target cells by NK cells.

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon™ BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50-nm) filter set. BD Horizon™ BV510 conjugates are useful for the detection of dim markers off the violet laser.

Specifications

• Antigen: CD69
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: H1.2F3
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Violet 510
• Description: VEA; Very Early Activation Antigen; AIM; Activation Induced Molecule
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Host Species: Hamster
• Immunogen: Mouse Dendritic Epidermal T Cell Line Y245
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Mouse
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG1