CD8 Mouse anti-Human, PE, Clone: 17D8, Invitrogen
Mouse Monoclonal Antibody
Manufacturer: Invitrogen MA112030
FACS procedure using PBMCs: Add 10 µL of MA1-12030/10^6 PBMC in 100 µL PBS. Mix gently and incubate for 15 minutes on ice. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. FACS procedure using whole blood: Add 10 µL of MA1-12030/100 µL of whole blood. Mix gently and incubate for 15 minutes at room temperature. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.CD8 (Cluster of Differentiation 8) is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediate efficient cell-cell interactions within the immune system. The CD8 antigen acts as a co-receptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 is found on a T cell subset of normal cytotoxic/suppressor cells which make up approximately 20-35 % of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer (NK) cells, subpopulations of peripheral blood null cells, thymocytes and bone marrow cells. The CD8 co-receptor functions as either a homodimer composed of two alpha chains, or as a disulfide-linked heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. The majority of CD8+ T cells express CD8 as a alpha/beta heterodimer. In HIV, the HIV-2 envelope glycoprotein binds CD8 alpha chain (but not the beta chain).
|human peripheral blood T lymphocytes|
|PBS with 0.2% carrier protein and 0.08% sodium azide|
|CD8, Leu-2, MAL, p32, CD8a, CD8b, CD8alpha, CD8beta|
|4° C, do not freeze|
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