CD8a Rat anti-Mouse, Unlabeled, Clone: 53-6.7, BD

Rat Monoclonal Antibody

Supplier:  BD Biosciences 550281

Catalog No. BDB550281

Description

The 53-6.7 antibody reacts with the 38kDa α and 34kDa α′ chains of the CD8 differentiation antigen (Ly-2 or Lyt-2) of all mouse strains tested. The CD8 α and α′ chains (CD8a) form heterodimers with the CD8 β chain (CD8b, Ly-3, or Lyt-3) on the surface of most thymocytes. A subpopulation of mature T lymphocytes (i.e., MHC class I-restricted T cells, including most T suppressor/cytotoxic cells) expresses almost exclusively the CD8 αβ heterodimer (the α′ chain is absent). Subsets of γδ TCR-bearing T cells, intestinal intrapithelial lymphocytes, and dendritic cells express CD8a without CD8b. It has been suggested that the expression of the CD8a/CD8b heterodimer is restricted to T lymphocytes which matured in the thymus or in an extrathymic environment that had been influenced by thymus-initiated neuroendocrine signals. CD8 is an antigen corecptor on the T-cell surface which interacts with MHC class I molecules on antigen-presenting cells or epithelial cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase Ick (p56 [lck]). The CD8 α and α′ chains arise from alternatively spliced messengers of a single CD8a gene. The longer α form associates with p56 [lck] via a CXCP motif in its cytoplasmic domain, which it shares with CD4, but not with CD8b. The truncated α' chain is unable to associate with p56
[lck], and it may function to attenuate the CD8-mediated costimulatory signal during intrathymic T-cell maturation. In vivo and in vitro treatment with 53-6.7 mAb has reportedly been effective at depleting CD8+ peripheral T lymphocytes. The 53-6.7 antibody has also been reported to cross-reaact with CD8 α- and α′-like polypeptides on subsets of thymic and peripheral lymphocytes in the Egyptian toad, Bufo AC93regularis.

Host Species: Rat
Clone: 53-6.7
Isotype: IgG2a κ
Species Reactivity: Mouse
Immunogen: Mouse thymus / spleen

Immunohistochemistry, Immunohistochemistry (Frozen)

Specifications

• Antigen: CD8a
• Classification: Monoclonal
• Concentration: 62.5μg/mL
• Description: Cd8a; CD8 alpha chain; Ly-2; Lyt2; Lyt-2; Ly-35; Ly-B
• Host Species: Rat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C

• Applications: Flow Cytometry
• Clone: 53-6.7
• Conjugate: Unconjugated
• Formulation: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
• Immunogen: Mouse thymus / spleen
• Quantity: 1 mL
• Research Discipline: Immunology
• Target Species: Mouse
• Isotype: IgG2a κ

For Research Use Only.