CD8b.2 Rat anti-Mouse, Biotin, Clone: 53-5.8, BD

Rat Monoclonal Antibody

Supplier:  BD Biosciences 553039

Catalog No. BDB553039

Description

The 53-5.8 antibody reacts with the β chain of the CD8 differentiation antigen (Ly-3.2 or Lyt-3.2) of most mouse strains, having weak reactivity with Ly-3.1 strains (e.g., AKR, C58, MRL, PL). The CD8 α and α' chains (CD8a) form heterodimers with the CD8 β chain (CD8b, Ly-3, or Lyt-3) on the surface of most thymocytes. A subpopulation of mature T lymphocytes (i.e., MHC class I-restricted T cells, including most T suppressor/cytotoxic cells) expresses almost exclusively the CD8 αβ heterodimer (the α' chain is absent). Subsets of γδ TCR-bearing T cells, intestinal intraepithelial lymphocytes, and dendritic cells express CD8a without CD8b. It has been suggested that the expression of the CD8a/CD8b heterodimer is restricted to T lymphocytes which matured in the thymus or in an extrathymic environment that had been influenced by thymus-initiated neuroendocrine signals. CD8 is an antigen coreceptor on the T-cell surface which interacts with MHC class I molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck (p56lck). This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Host Species: Rat
Clone: 53-5.8
Isotype: IgG1 κ
Species Reactivity: Mouse
Immunogen: Mouse Thymus / Spleen

Flow Cytometry

Specifications

• Antigen: CD8b.2
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Description: Ly-3.2
• Host Species: Rat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: 53-5.8
• Conjugate: Biotin
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Mouse Thymus / Spleen
• Quantity: 0.5 mg
• Research Discipline: Immunology
• Target Species: Mouse
• Isotype: IgG1 κ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only.