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CD90.2 Rat anti-Mouse, Brilliant Violet 786, Clone: 53-2.1, BD

Rat Monoclonal Antibody

Manufacturer:  BD Biosciences 564365

Catalog No. BDB564365


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Description

Description

The 53-2.1 monoclonal antibody specifically binds to the CD90.2 (Thy-1.2) alloantigen on thymocytes, most peripheral T lymphocytes, some intraepithelial T lymphocytes (IEL, DEC), epithelial cells, fibroblasts, neurons, hematopoietic stem cells, but not B lymphocytes, of most mouse strains. The 53-2.1 antibody has been reported not to crossreact with Thy-1.1 (e.g., AKR/J, PL), or with rat Thy-1. CD90 is a glycosyolphosphatidylinositol-anchored membrane glycoprotein of the Ig superfamily that is involved in signal transduction. In addition, there is evidence that CD90 mediates adhesion of thymocytes to thymic stroma. The 53-2.1 antibody has been reported to block the binding of anti-mouse CD90.2 clone 30-H12 (Cat. No. 553009) to immobilized thymocyte membranes.

The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 786-nm. BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60-nm filter).

Specifications

Specifications

CD90.2
53-2.1
Brilliant Violet 786
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Rat
IgG2a, κ
RUO
Primary
Murine
Flow Cytometry
0.2mg/mL
Thy-1.2; T25; Thymus cell antigen 1, theta
Affinity Purified
Mouse Thymus / Spleen
50μg
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
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