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CD90.2 Rat anti-Mouse, Brilliant Violet 605, Clone: 53-2.1, BD

Catalog No. BDB563008 Shop All BD Biosciences Products
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BDB563008 50 μg
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Catalog No. BDB563008 Supplier BD Biosciences Supplier No. 563008
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Description

Rat Monoclonal Antibody

The 53-2.1 monoclonal antibody specifically binds to the CD90.2 (Thy-1.2) alloantigen on thymocytes, most peripheral T lymphocytes, some intraepithelial T lymphocytes (IEL, DEC), epithelial cells, fibroblasts, neurons, hematopoietic stem cells, but not B lymphocytes, of most mouse strains. The 53-2.1 antibody has been reported not to crossreact with Thy-1.1 (e.g., AKR/J, PL), or with rat Thy-1. CD90 is a glycosyolphosphatidylinositol-anchored membrane glycoprotein of the Ig superfamily that is involved in signal transduction. In addition, there is evidence that CD90 mediates adhesion of thymocytes to thymic stroma. The 53-2.1 antibody has been reported to block the binding of anti-mouse CD90.2 clone 30-H12 (Cat. No. 553009) to immobilized thymocyte membranes.

This antibody is conjugated to BD Horizon BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.

TRUSTED_SUSTAINABILITY

Specifications

Antigen CD90.2
Applications Flow Cytometry
Classification Monoclonal
Clone 53-2.1
Concentration 0.2mg/mL
Conjugate Brilliant Violet 605
Description Thy-1.2; T25; Thymus cell antigen 1, theta
Formulation Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Host Species Rat
Immunogen Mouse Thymus / Spleen
Purification Method Affinity Purified
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Mouse
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG2a κ
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