CD90.2 Rat anti-Mouse, FITC, Clone: 30-H12, BD

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
BDB553012	BD Biosciences 553012	0.1 mg
BDB553013	BD Biosciences 553013	0.5 mg

Description

The 30-H12 clone has been reported to react with the CD90.2 (Thy-1.2) alloantigen on thymocytes, most peripheral T lymphocytes, some intraepithelial T lymphocytes (IEL, DEC), epithelial cells, fibroblasts, neurons, hematopoietic stem cells, but not B lymphocytes, of most mouse strains. Thy-1.2 has also been initially reported to be detectable on thymic dendritic cells, but later revealed that the antigen was probably picked up from T-lineage cells. 30-H12 mAb has been reported not to cross-react with Thy-1.1 (e.g., AKR/J, PL), or with rat Thy-1. CD90 is a GPI-anchored membrane glycoprotein of the Ig superfamily which is involved in signal transduction. In addition, there is evidence that CD90 mediates adhesion of thymocytes to thymic stroma. It has been reported that crosslinked 30-H12 antibody induces Ca2+ influx into thymocytes and that co-crosslinking of 30-H12 mAb with antibody to the CD3/TCR complex intensifies thymocyte signal transduction, promotes apoptosis of thymocytes, and inhibits the CD3-mediated proliferative response of mature T lymphocytes. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Host Species: Rat
Clone: 30-H12
Isotype: IgG2b κ
Species Reactivity: Mouse
Immunogen: Mouse Thymus / Spleen

Flow Cytometry

Specifications

• Antigen: CD90.2
• Concentration: 0.5mg/mL
• Classification: Monoclonal
• Host Species: Rat
• Research Discipline: Immunology
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Isotype: IgG2b κ
• Purification Method: Affinity Purified
• Description: Thy-1.2

• Clone: 30-H12
• Applications: Flow Cytometry
• Conjugate: FITC
• Regulatory Status: RUO
• Target Species: Mouse
• Immunogen: Mouse Thymus / Spleen
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

For Research Use Only.