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CD93 (Early B Lineage) Rat anti-Mouse, PE-CF594, Clone: AA4.1, BD

Catalog No. BDB563805 Shop All BD Biosciences Products
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BDB563805 50 μg
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Catalog No. BDB563805 Supplier BD Biosciences Supplier No. 563805

Description

Rat Monoclonal Antibody

The AA4.1 monoclonal antibody specifically recognizes the Early B Lineage antigen which is also known as CD93, AA4 antigen, Ly-68, and Complement component C1q receptor (C1qRp). This 130-140-kDa type I transmembrane glycoprotein is expressed on immature B lymphocytes in the adult bone marrow and onhematopoietic progenitors and stem cells in adult bone marrow, fetal liver, and embryonic yolk sac. Although CD93+ cells are most plentiful in adult mouse bone marrow, a smaller number of CD93+ cells which express lower CD93 levels can be detected in the adult spleen using bright fluorescent conjugates of the AA4.1 antibody or an amplified indirect immunofluorescent staining procedure. It has been observed that the staining pattern of the 493 monoclonal antibody is similar to that of the AA4.1 antibody, that both antibodies precipitate molecules of the same molecular weight, and that staining with the AA4.1 antibody is not blocked by the 493 antibody. These results suggest that the antibodies recognize separate epitopes on the same Early B Lineage antigen.

This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red™. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red™ yet exhibits improved brightness and spectral characteristics. Due to PE having maximal absorption peaks at 496nm and 564nm, PE-CF594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red™ (eg, 610/20-nm filter).

TRUSTED_SUSTAINABILITY

Specifications

Antigen CD93 (Early B Lineage)
Applications Flow Cytometry
Classification Monoclonal
Clone AA4.1
Concentration 0.2mg/mL
Conjugate PE-CF594
Description Aa4; Cd93; C1qRp; C1qr1; Ly-68; Ly68; C1q/MBL/SPA receptor
Formulation Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Host Species Rat
Immunogen Pre-B lymphoma 70Z/3, derived from (C57BL/6 x DBA/2)F1 mouse
Purification Method Affinity Purified
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Mouse
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG2b κ
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