MilliporeSigma™ anti-CD99 Clone: O662,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABF927

Catalog No. MABF927MI

Description

Specifically detects CD99 Clone: O662 in Human samples, and it is validated for Activity Assay, Flow Cytometry, Western Blotting

CD99 antigen (UniProt P14209; also known as 12E7, E2 antigen, HBA-71 antigen, Protein MIC2, T-cell surface glycoprotein E2, Surface antigen MIC2) is encoded by the CD99 (also known as HBA71, MIC2, MIC2X, MIC2Y, MSK5X) gene (Gene ID 4267) in human. Originally identified as a thymus-leukemia-associated antigen, CD99 is a transmembrane glycoprotein expressed on many hematopoietic and non-hematopoietic cell types. CD99 is involved in cellular adhesion and migration as well as in survival. Antibody-mediated cross-linking of cell surface CD99 is shown to induce apoptotic signals in Jurkat T cells and essential thrombocythemia (ET) cell lines in vitro, and several CD99 monoclonal antibodies show in vivo efficacy in ET mouse models. CD99 is initially produced with a signal peptide sequence (a.a. 1-22), the removal of which yields the mature CD99 with a large extracellular domain (a.a. 23-122), followed by a transmembrane segment (a.a. 123-147) and a short cytoplasmic tail (a.a. 148-185).

Specifications

• Antigen: CD99
• Classification: Monoclonal
• Conjugate: Unconjugated
• Gene Accession No.: P14209
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Clone 0662 detects both the 32kDa glycosylated (plasma membrane) and the 28kDa non-glycosylated (intracellular) forms of human CD99/E2 (Gelin, C., et al. (1989). EMBO J. 8(11):3253-3259). Note that the clone was originally designated as 0662 (zero six six two) by Bernard, A., et al. (1988), but subsequently cited in some publications as O662 with a captial letter O.
• Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
• Isotype: IgG3 κ

• Applications: Functional Assay, Flow Cytometry, Western Blot
• Clone: O662
• Formulation: Purified mouse monoclonal IgG3κ antibody in PBS without preservatives.
• Gene Symbols: CD99, HBA71, MIC2, MIC2X, MIC2Y, MSK5X
• Immunogen: Human thymocytes.
• Quantity: 100 μg
• Research Discipline: Inflammation, Immunology
• Gene ID (Entrez): NP_001116370
• Target Species: Human
• Form: Purified

For Research Use Only