Cdc25C Mouse anti-Human, Unlabeled, Clone: C2-2, BD

Catalog No.	Quantity
BDB556576	0.1 mg

Catalog No. BDB556576 Supplier BD Biosciences Supplier No. 556576

Description

Mouse Monoclonal Antibody

Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell-cycle control in eukaryotes. Cyclins contain a conserved amino acid sequence motif, the cyclin box, through which they bind to cdks to form active complexes that regulate the progression of the cell cycle. These complexes are regulated by activating and inhibitory phosphorylation events, as well as by interactions with small regulatory proteins including p21 and p27Kip1. Cdc25A, Cdc25B and Cdc25C are a family of phosphatases that activate cyclin-cdk complexes at different checkpoints of the cell cycle. Cdc25C appears to be active during G2 where it controls entry into mitosis by dephosphorylating and thus activating the cdk1 component of cyclin B1-cdk1 complexes. In response to DNA damage, Cdc25C becomes phosphorylated by the recently identified kinases Chk1 and c-TAK1 creating a binding site for 14-3-3 proteins which inhibits the phosphatase activity of Cdc25 proteins. Thus Cdc25C serves a dual purpose during the cell cycle via its ability to promote G2/M transition in normally cycling cells as well as by providing a cell cycle checkpoint in response to DNA damage. Multiple electrophoretic forms of Cdc25C have been reported and are thought to result from differences in the phosphorylation state of the Cdc25C protein. Full length, recombinant human Cdc25C was used as immunogen. HeLa human cervical carcinoma (ATCC CCL-2) and A549 human lung carcinoma (ATCC CCL-185) cells are suggested as positive controls for the western blot application. This antibody is routinely tested by Western blot analysis and immunofluorescent imaging. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Host Species: Mouse
Clone: C2-2
Isotype: IgG1
Cross Reactivity: Human

Bioimaging, Western Blotting

Specifications

• Antigen: Cdc25C
• Applications: In vitro Assay, Western Blot
• Classification: Monoclonal
• Clone: C2-2
• Concentration: 0.5mg/mL
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Affinity Purified
• Quantity: 0.1 mg
• Regulatory Status: RUO
• Research Discipline: Cell Biology
• Primary or Secondary: Primary
• Target Species: Human
• Isotype: IgG

For Research Use Only.