Cdk2 with control Rabbit, Unlabeled, Polyclonal, BD

Rabbit Polyclonal Antibody

Supplier:  BD Biosciences 558896

Catalog No. BDB558896

Description

Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell cycle control in eukaryotes. Cdks are catalytic subunits whose activity requires interaction with their regulatory subunits, the cyclins, as well as specific phosphorylation events. The precise timing of cyclin-cdk activity during the cell cycle determines whether the cell cycle continues or becomes blocked. Cdks 2, 4, 5, and 6 may associate with D-type cyclins. Interaction between cdk2 and cyclin E during G1/S transition creates a complex with histone H1 kinase activity. This complex is thought to be required for the initiation and progression of DNA replication during S phase. Cdk2-cyclin A complexes appear during late S phase and also play a role in progression of DNA replication. Substrates for cdk-cyclin complexes include nuclear lamins, histones, oncogenes, (c-src, c-abl, SV40 large-T), tumor suppressor genes (e.g., RB and p53), nucleolin, RNA polymerase II and others. Cdk2 migrates at ∽33kDa on SDS-PAGE. The polyclonal antibodies recognize human and mouse cdk2. The antibodies do not react with other known cdk proteins. A synthetic peptide corresponding to amino acids 287-298 (QDVTKPVPHLRL) at the C-terminus of human cdk2, with the addition of a C-terminal cysteine residue (C) to facilitate coupling to KLH, was used as immunogen. The polyclonal antibodies recognize human and mouse cdk2. The antibodies do not react with other known cdk proteins. A synthetic peptide corresponding to amino acids 287-298 (QDVTKPVPHLRL) at the C-terminus of human cdk2, with the addition of a C-terminal cysteine residue (C) to facilitate coupling to KLH, was used as immunogen.

Immunoprecipitation, Western Blotting

Specifications

• Antigen: Cdk2 with control
• Classification: Polyclonal
• Conjugate: Unconjugated
• Immunogen: Human cdk2 aa. 287-298 synthetic peptide
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Form: Serum

• Applications: Immunoprecipitation, Western Blot
• Clone: POLYCLONAL
• Host Species: Rabbit
• Quantity: 0.1 mL
• Research Discipline: Cell Biology
• Target Species: Human, Mouse
• Isotype: IgG