Cdk4 (N-Term) Mouse anti-Human, Unlabeled, Clone: DCS-35, BD

Catalog No.	Quantity
BDB559693	0.1 mg

Catalog No. BDB559693 Supplier BD Biosciences Supplier No. 559693

Description

Mouse Monoclonal Antibody

Cyclins, cyclin-dependent kinases (Cdks), and cyclin-dependent kinase inhibitors (CdkIs) are essential for cell-cycle control in eukaryotes (reviewed in 1). Cyclins, regulatory subunits, bind to cyclin-dependent kinases (Cdks), catalytic subunits, to form active cyclin-Cdk complexes. Cdk subunits by themselves are inactive and binding to a cyclin is required for their activity. Cyclins A, B1, D and E undergo periodic synthesis and degradation, thereby providing a mechanism to regulate Cdk activity throughout the cell cycle. Additionally, Cdk activity is further regulated by activating and inhibitory phosphorylations, and small proteins (p15, p16, p18, p19, p21 and p27), called CdkIs, that bind to cyclins, Cdks, or cyclin-Cdk complexes. Cdk4 was originally called PSK-J3,2 and following demonstration of its association with D-type cyclins, became known as Cdk4.2 D-type cyclins also associate with Cdks 2 and 5, although Cdk4 appears to be the most abundant partner. The D-type cyclins (D1, D2, and D3) are expressed in response to growth factors or mitogens, and rapidly degrade when mitogens are withdrawn. D cyclins appear to promote G0 to G1 transitions and the rate of G1 progression. For example, cyclin D-Cdk4 and cyclin D-Cdk6
complexes phosphorylate the retinoblastoma protein (Rb) which removes the G1 phase block caused by underphosphorylated Rb. Cdk4 has a molecular weight of ∽32kDa.
This antibody is routinely tested by western blot and bioimaging analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Immunofluorescence, Immunoprecipitation, Western Blotting

Specifications

• Antigen: Cdk4 (N-Term)
• Applications: Western Blot
• Classification: Monoclonal
• Clone: DCS-35
• Concentration: 0.5mg/mL
• Conjugate: Unconjugated
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Mouse
• Immunogen: Human Cdk4
• Purification Method: Affinity Purified
• Quantity: 0.1 mg
• Regulatory Status: RUO
• Research Discipline: Cell Biology
• Primary or Secondary: Primary
• Target Species: Canine, Human, Mouse, Rat
• Content And Storage: Store undiluted at 4°C
• Isotype: IgG1 κ