MilliporeSigma™ anti-CEP Clone: 7E12H12,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABC530

Catalog No. MABC530MI

Description

Specifically detects CEP Clone: 7E12H12 in Human samples, and it is validated for Dot Blot, ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blotting

The Monoclonal (mAb) Das-1 was originally desiganted as clone 7E12H12 and was thought to target a 40kDa colon-specific tropomyosin (TM) isoform or a TM-related protein. It was later found that this clone specifically immunoprecipitated a high molecular weight chaperone protein that targets tropomyosin 5 (TM5) in human colon epithelial cells and subsequently named Colon Epithelial Protein (CEP). The Das-1 target is a glycoprotein with variable apparent sizes depending on the level of glycosylation. The target glycoprotein immunoprecipitated from the plasma membrane of DLD-1 colon cancer cells appears with an apparent molecular size of 185kDa, while the target isolated from LS-180 colon cancer cells migrates with a size range extends to greater than 200kDa. Das-1/CEP immunoreactivity is reported to be associated with Barrett's metaplasia, Barrett's adenocarcinoma, as well as with gastric adenocarcinomas and carcinoma-associated intestinal metaplasia, but no reactivity has been found in normal esophageal, gastric or small intestinal mucosa. When tested by ELISA and imunoblotting against recombinant human tropomyosins isoforms 1-5, none of five hTM isoforms reacted with mAB DAS-1. As determined by immunofluorescence and FACS analyses, CEP/Das-1 immunoreactivity is detected in several colon cancer lines (DLD-1, LS180, T84), but not in the colorectal adenocarcinoma cell line HT-29. CEP/Das-1 immunoreactivity is not detected among non-colonic epithelial cell lines (293-T, HeLa, pancreatic cancer) or hematopoietic lines (K562, KGI, Daudi).

Specifications

• Antigen: CEP
• Classification: Monoclonal
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Purified by ion-exchange chromatography
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Form: Purified

• Applications: Dot Blot, ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Clone: 7E12H12
• Formulation: Purified mouse monoclonal IgMκ antibody in PBS with 0.05% sodium azide.
• Immunogen: Human colonic protein extract.
• Quantity: 100 μL
• Research Discipline: Apoptosis, Cancer
• Test Specificity: Clone 7E12H12 (mAb DAS-1) specifically reacts with Colon Epithelial Protein (CEP), but not human tropomyosins isoforms (hTM1-5). CEP/Das-1 immunoreactivity is detected in several colon cancer lines (DLD-1, LS180, T84), but not in the colorectal adenocarcinoma cell line HT-29. CEP/Das-1 immunoreactivity is not detected among non-colonic epithelial cell lines (293-T, HeLa, HUVEC, pancreatic cancer) or hematopoietic lines (K562, KGI, Daudi).
• Content And Storage: 2°C to 8°C for one year from date of shipment
• Isotype: IgM κ

For Research Use Only