Clec12A Rat anti-Mouse, Brilliant Violet 421, Clone: 5D3 (also known as 1/06-5D3), BD

Rat Monoclonal Antibody

Manufacturer:  BD Biosciences 564795

Catalog No. BDB564795

Description

The 5D3 monoclonal antibody specifically binds to mouse C-type lectin domain family 12 member A (Clec12A), also known as myeloid inhibitory C-type lectin-like receptor (MICL), C-type lectine-like molecule 1 (CLL-1) or Dendritic cell-associated lectin 2 (DCAL-2). Clec12A is expressed on plasmacytoid dendritic cells, subsets of myeloid dendritic cells, monocytes, macrophage and B cells. Clec12A has one single C-type lectin-like domain and one cytoplasmic immunoreceptor tyrosine-based inhibitory motif. Clec12A reportedly serves as a negative regulator of granulocyte and monocyte function.

The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

Specifications

• Antigen: Clec12A
• Clone: 5D3 (also known as 1/06-5D3)
• Conjugate: Brilliant Violet 421
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Host Species: Rat
• Isotype: IgG_2a, κ, also known as Wistar (outbred) IgG_2a, κ
• Quantity: 50μg
• Storage Requirements: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Monoclonal or Polyclonal: Monoclonal

• Applications: Flow Cytometry
• Concentration: 0.2mg/mL
• Description: CL12A; C-type lectin domain family 12 member A; CLL-1; DCAL-2; KLRL1; MICL
• Format: Affinity Purified
• Immunogen: Mouse Clec12A Transfected Cell Line
• Purification Method: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Murine