The csk gene encodes a protein kinase that phosphorylates several Src family kinases including p60(c-src), p59(fyn), p53/56(lyn), p56(lck), and p62(yes). This phosphorylation occurs specifically at the carboxy-terminal tyrosine, Y527. Csk (c-src kinase) was identified based on its ability to phosphorylate exogenous Src in mammalian src-deficient cells. Establishment of a mouse csk- cell line revealed that phosphorylation of Y527 in Src was reduced 20-50%. This correlated with an increase of Src kinase activity, thus indicating Csk is a negative regulator of src. Csk-deficient cells also show an elevation in the kinase activity of p59(fyn) and p53/56(lyn). These data suggest that Csk may be a universal negative regulator of Src family kinases in vivo.
Host Species: Mouse
Species Reactivity: Mouse
Immunogen: Rat Csk aa. 1-156
Formula Weight [Chemical]: 50kDa
Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
|Rat Csk aa. 1-156|
|Store undiluted at -20°C.|
|Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.|
For Research Use Only.
We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.
Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.Ok