MilliporeSigma™ anti-Cullin-4A/CUL4A Clone: 2A2.1,

Catalog No.	Quantity	Conjugate
MABC554MI	100 μg	Unconjugated

Catalog No. MABC554MI Supplier MilliporeSigma™ Supplier No. MABC554

Description

Mouse Monoclonal Antibody

Specifically detects Cullin-4A/CUL4A Clone: 2A2.1 in Human samples, and it is validated for Western Blotting

Cullin-4A (UniProt Q13619; also known as CUL-4A) is encoded by the CUL4A gene (Gene ID 8451) in human. Polyubiquitination of proteins is mediated by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). There exist RING finger-type (really interesting new gene), HECT-type (homologous to E6-AP C-terminus), and U-box type of E3 ligases. Cullin-4 belongs to the cullin family of hydrophobic proteins that confer substrate specificity to the multimeric Cullin-RING E3 ubiquitin ligase (CRL) complexes by acting as scaffold proteins. The human genome encodes 8 cullin proteins (CUL 1, 2, 3, 4A, 4B, 5, 7 and 9). The activity of CRLs is regulated by Cullin Neddylation via the reversible conjugation of a small ubiquitin-type protein known as Neural precursor cell expressed developmentally down-regulated protein 8 (Nedd8). CUL4A interacts with the substrate-binding adaptor DDB1 (DNA damage binding protein 1) via its arc-shaped helical N-terminal domain, and binds the small RING finger protein ROC1 (regulator of cullins 1) via its globular C-terminal domain. Despite the 83% sequence identity and seemingly functional redundancy between CUL4A and CUL4B, much more focus is placed on CUL4A in oncogenesis research.

Specifications

• Antigen: Cullin-4A/CUL4A
• Applications: Western Blot
• Classification: Monoclonal
• Clone: 2A2.1
• Conjugate: Unconjugated
• Formulation: Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
• Gene Accession No.: Q13619
• Gene Symbols: CUL4A
• Host Species: Mouse
• Immunogen: KLH-conjugated linear peptide corresponding to human Cullin-4A/CUL4A near the C-terminus.
• Purification Method: Protein G purified
• Quantity: 100 μg
• Regulatory Status: RUO
• Research Discipline: Apoptosis, Cancer
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_001008895
• Test Specificity: Clone 2A2.1 is expected to react with both spliced isoforms of human cullin-4A
• Target Species: Human
• Content And Storage: 2°C to 8°C for one year from date of shipment
• Form: Purified
• Isotype: IgG1 κ

For Research Use Only