CXCR5 Rat anti-Human, BB515, Clone: RF8B2, BD

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
BDB564625	BD Biosciences 564625	25 Tests
BDB564624	BD Biosciences 564624	100 Tests

Description

The RF8B2 monoclonal antibody specifically binds to the human CXC chemokine receptor, CXCR5. CXCR5 (also known as CD185, BLR-1 NLR and MDR15), a seven transmembrane, G-protein-coupled receptor, is the specific receptor for CXC chemokine, CXCL13/BLC/BCA-1. In peripheral blood, CXCR5 expression is restricted to B lymphocytes and a small subset of CD4+ and CD8+ lymphocytes. The restricted expression pattern of CXCR5 on B cells and follicular T helper cells (Tfh) suggests that this receptor functions as a regulator of B and T cell migration. Stimulation of T cells with anti-CD3 monoclonal antibody leads to the down-regulation of CXCR5.

The antibody was conjugated to BD Horizon BB515 which was developed exclusively by BD Biosciences. With an excitation max of 490nm and an emission max of 515nm, BD Horizon BB515 can be excited by the 488nm laser and detected in a standard FITC set (eg, 530/30-nm filter). This dye provides a much brighter alternative to FITC with less spillover into the PE detector.

Specifications

• Antigen: CXCR5
• Applications: Flow Cytometry
• Conjugate: Brilliant Blue 515 (BB515)
• Regulatory Status: RUO
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide
• Isotype: IgG2b κ
• Purification Method: Affinity Purified
• Description: CD185; Chemokine C-X-C motif receptor 5; BLR1; MDR-15

• Clone: RF8B2
• Classification: Monoclonal
• Host Species: Rat
• Target Species: Human
• Immunogen: Human CXCR5
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

For Research Use Only.