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CXCR5 Rat anti-Mouse, BUV395, Clone: 2G8, BD

Rat Monoclonal Antibody

Manufacturer:  BD Biosciences 563980

Catalog No. BDB563980

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The 2G8 monoclonal antibody specifically binds to the mouse CXC chemokine receptor, CXCR5. CXCR5 is also known as CD185, BLR1, NLR and MDR15. CXCR5 is a seven-transmembrane, G-protein-coupled receptor that is specific for the CXC chemokine, CXCL13/BLC/BCA-1. The expression of CXCR5 has been detected in spleen, lymph nodes, tonsils, brain, bone marrow, T cells, B cells, cerebrum, cerebellum, hippcampus and pituitary. In mouse spleen, CXCR5 was strictly expressed by mature B cells and a small subset of T lymphocytes. CXCR5 plays a role in directing the migration of B and T cells to B cell follicles with the spleen and certain other lymphoid tissues. The immunogen used to generate 2G8 hybridoma was a recombinant protein containing N-terminal amino acids of mouse CXCR5 (GST-NmBLR1).

The antibody was conjugated to BD Horizon™ BUV395 which has been exclusively developed by BD Biosciences as an optimal dye for use on a 355nm laser equipped instrument. With an Ex Max at 348 nmand an Em Max at 395nm, this dye has virtually no spillover into any other detector. BD Horizon BUV395 can be excited with a 355nm laser and detected with a 379/28 filter.



Brilliant Ultraviolet 395
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
IgG2a, κ
Flow Cytometry
CD185; CXC-R5; CXCR-5; C-X-C chemokine receptor type 5; Blr1; MDR15; Gpcr6
Affinity Purified
Mouse CXCR5
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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