Cytokeratin Mouse anti-Human, Brilliant Violet 421, Clone: CAM5.2, BD

Catalog No.	Quantity
BDB564709	50 Tests

Catalog No. BDB564709 Supplier BD Biosciences Supplier No. 564709

Description

Mouse Monoclonal Antibody

The CAM5.2 monoclonal antibody specifically recognizes cytokeratin having a primary reactivity with human keratin proteins that correspond to Moll's peptides #7 (48 kDa) and #8 (52 kDa). Cytokeratin 7 and 8 are type II cytoskeletal keratins. These cytoskeletal proteins provide structural integrity for epithelial cells and may serve other functions as well. They are expressed in epithelia cells that comprise normal human tissues. Although these cytokeratins are not normally expressed in stratified squamous epithelium, they may be expressed in some squamous cell carcinomas. The CAM 5.2 antibody stains most epithelial-derived tissue, including liver, renal tubular epithelium, and hepatocellular and renal cell carcinomas.

The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

Specifications

• Antigen: Cytokeratin
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: CAM5.2
• Conjugate: Brilliant Violet 421
• Description: Cytokeratin-7/-8; CK-7/CK-8;
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Host Species: Mouse
• Immunogen: Human HT29 colorectal carcinoma cell line
• Purification Method: Affinity Purified
• Quantity: 50 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG2a