Cytokeratin Mouse anti-Human, Brilliant Violet 510, Clone: CAM 5.2, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 563613

Catalog No. BDB563613

Description

The CAM5.2 monoclonal antibody specifically recognizes cytokeratin having a primary reactivity with human keratin proteins that correspond to Moll's peptides #7 (48 kDa) and #8 (52 kDa). Cytokeratin 7 and 8 are type II cytoskeletal keratins. These cytoskeletal proteins provide structural integrity for epithelial cells and may serve other functions as well. They are expressed in epithelia cells that comprise normal human tissues. Although these cytokeratins are not normally expressed in stratified squamous epithelium, they may be expressed in some squamous cell carcinomas. The CAM 5.2 antibody stains most epithelial-derived tissue, including liver, renal tubular epithelium, and hepatocellular and renal cell carcinomas.

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon™ BV510 can be excited by the violet laser and detected in the BD Horizon™ V500 (525/50-nm) filter set. BD Horizon™ BV510 conjugates are useful for the detection of dim markers off the violet laser.

Specifications

• Antigen: Cytokeratin
• Classification: Monoclonal
• Conjugate: Brilliant Violet 510
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Immunogen: Human HT29 colorectal carcinoma cell line
• Quantity: 50 Tests
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: CAM 5.2
• Description: Cytokeratin-7/-8; CK-7/CK-8;
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Human
• Isotype: IgG2a