DAP Kinase Mouse anti-Human, Unlabeled, Clone: 17, BD

Catalog No.	Quantity
BDB610291	150 μg

Catalog No. BDB610291 Supplier BD Biosciences Supplier No. 610291

Description

Mouse Monoclonal Antibody

Chronic exposure to extracellular signals such as interferons induce the inhibition of cell proliferation followed by cell death. The gene for DAP Kinase (Death Associated Protein Kinase) was identified using a novel approach named Technical Knockout. Briefly, Hela cells were transfected with an antisense cDNA expression library, then exposed to Interferon-γ. The surviving cells, with their antisense cDNAs, were rescued and the “protecting” genes isolated. The DAP Kinase gene encodes a protein of 1423 amino acids, a molecular weight of 160kDa, a kinase domain at its amino terminal region, ankyrin repeats in the middle region, and a death domain at the extreme C-terminus. DAP Kinase phosphorylates at Ser/Thr residues in a Ca²⁺/Calmodulin-dependent fashion. It has been demonstrated that Ca²⁺/Calmodulin binds directly to DAP Kinase at its amino terminal region. In addition, immunostaining studies localized DAP Kinase in association with the actin filaments where it may phosphorylate myosin light chain. Thus, this novel cytoskeletal and Ca²⁺/Calmodulin-dependent protein kinase plays a role in interferon-γ-induced cell death.

Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting

Specifications

• Antigen: DAP Kinase
• Applications: Western Blot
• Classification: Monoclonal
• Clone: 17
• Concentration: 250μg/mL
• Conjugate: Unconjugated
• Formulation: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Host Species: Mouse
• Immunogen: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Purification Method: Affinity Purified
• Quantity: 150 μg
• Regulatory Status: RUO
• Research Discipline: Cell Biology
• Primary or Secondary: Primary
• Target Species: Human
• Content And Storage: Store undiluted at -20°C.
• Isotype: IgG1

For Research Use Only.