Dendritic Cells Rat anti-Mouse, Biotin, Clone: 33D1, BD

Rat Monoclonal Antibody

Supplier:  BD Biosciences 552776

Catalog No. BDB552776

Description

The 33D1 antibody reacts with an antigen on most dendritic cells (DC) of spleen, lymph node, and Peyer's patch, but not liver, bone marrow, or epidermal dendritic cells; macrophages; other leukocytes; or erythroid cells. Within the spleen, the majority of 33D1+ DC are locallized in the marginal zones. Thymic dendritic cells may express a low level of the 33D1 antigen. It has been reported that bone-marrow DC can be induced to express the 33D1 antigen by culture in the presence of GM-CSF, and the resulting 33D1+ DC are effective in in vitro (induction of MLR) and in vivo (anti-tumoral vaccination) assays for antigen presentation. However, the addition of IL-4 to GM-CSF in bone-marrow cultures resulted in loss of 33D1 expression and enhanced the MLR-stimulatory activity of the DC. It has also been reported that 33D1 expression is upregulated when liver-derived DC are cultured on collagen-coated plates in the presence of GM-CSF. In vivo functional 33D1+ DC are induced in the brains of mice chronically infected with Toxoplasma gondii, probably via the parasite's induction of GM-CSF.

Host Species: Rat
Clone: 33D1
Isotype: IgG2b κ
Species Reactivity: Mouse
Immunogen: Dendritic cells purified from mouse spleen and lymph node

Flow Cytometry, Immunofluorescence, Immunohistochemistry (Frozen)

Specifications

• Antigen: Dendritic Cells
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Host Species: Rat
• Quantity: 0.5 mg
• Research Discipline: Immunology
• Target Species: Mouse

• Applications: Flow Cytometry
• Clone: 33D1
• Conjugate: Biotin
• Immunogen: Dendritic cells purified from mouse spleen and lymph node
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Isotype: IgG2b

For Research Use Only.