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DNA Ligase III Mouse, Unlabeled, Clone: 7, BD

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 611876

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Catalog No. BDB611876


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Description

Description

Cells have evolved DNA repair pathways that are dedicated to the maintenance of DNA integrity. In such pathways, damaged DNA is excised and the resulting gap is filled by DNA polymerase. Human DNA ligases, ligase I, III, and IV, utilize ATP as a co-factor in DNA joining reactions required for base excision and single strand break repair pathways. All DNA ligases contain an RFPR sequence and an active site motif (ASM) on each side of their catalytic domain. The RFPR is required for transfer of an AMP group from the enzyme to the 5 ft.-phosphate terminus of a DNA nick. In addition, DNA ligase III has an N-terminal zinc finger domain (ZFD) that is homoloqous with the zinc fingers found in poly(ADP-ribose) polymerase (PARP). This domain is not required for DNA ligase activity, but enables DNA ligase III to interact with single strand gaps and single strand flaps. During base excision repair (BER), ATP-dependent ligation requires PARP, DNA polymerase β, and DNA ligase III interaction with XRCC1 within the BER complex. Thus, DNA ligase III may contain unique protein sequences that allow interaction and repair of specific types of DNA damage.

Host Species: Mouse
Clone: 7
Isotype: IgG1
Species Reactivity [for Features Main]: Human
Immunogen: Human DNA Ligase III aa. 2-115

Immunofluorescence, Western Blotting

Specifications

Specifications

DNA Ligase III
7
Unconjugated
Affinity Purified
Human DNA Ligase III aa. 2-115
50μg
Cell Biology
Primary
Canine, Human, Murine, Rat
Western Blot
250μg/mL
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
Murine
IgG1
RUO
Store undiluted at -20°C.
Monoclonal
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