MilliporeSigma™ anti-DNA-PK Clone: 18-2,

Catalog No.	Quantity	Conjugate
NA57100UG	100 μg	Unconjugated

Catalog No. NA57100UG Supplier MilliporeSigma™ Supplier No. NA57100UG

Description

Mouse Monoclonal Antibody

Specifically detects DNA-PK Clone: 18-2 in Human samples, and it is validated for Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Neutralization, Immuno Blotting

DNA Dependent Protein Kinase, DNA-PK, is a nuclear serine/threonine protein kinase composed of two subunits, a ∽470kDa catalytic subunit, DNA-PKcs, and the Ku autoantigen, the heterodimeric DNA binding protein p70/p80. DNA-PK has been shown to phosphorylate a number of key proteins in vitro including p53, Sp1, myc, RPA, and RNA Pol I. DNA-PK activity requires association of DNA-PKcs with Ku, DNA binding, and the presence of DNA ends. Mutations in DNA-PKcs contribute to murine SCID phenotypes which leads to inefficient V(D)J recombination in developing T & B lymphocytes. Loss of DNA-PK activity in fibroblasts or lymphocytes also confers on cells hypersensitivity to killing by ionizing radiation. These two phenotypes implicate DNA-PK in the repair of DNA double strand breaks in all cells and V(D)J recombination in lymphocytes. The 4127 amino acid DNA-PKcs protein shares a high degree of similarity with the ATM family of kinases, including ATM, ATR, and FRAP in humans and MEC1 and TEL1 in yeast. The catalytic domain of DNA-PKcs also shares homology with the PI3K family of lipid kinases and DNA-PK is inhibitable by wortmanin, a lipid kinase inhibitor. While the exact role for DNA-PK in cell cycle regulation is unclear, the protein may be important for suppression of apoptosis since DNA-PKcs is targeted for proteolysis during apoptosis, which leads to loss of catalytic activity. DNA-PK can also be inactivated through phosphorylation, possibly by c-abl and by autophosphorylation. DNA-PKcs is expressed in all cells and a DNA-PK activity is observed in all higher eukaryotes examined. The protein level of DNA-PKcs in human cells is very high, 50 to 100x that observed in murine cells.

Specifications

• Antigen: DNA-PK
• Applications: Immunoblot, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Neutralization
• Classification: Monoclonal
• Clone: 18-2
• Conjugate: Unconjugated
• Formulation: In 10mM PBS, 0.2% BSA, pH 7.4.
• Host Species: Mouse
• Immunogen: DNA-PK purified from HeLa cells
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes the ∽470kDa DNA-PK protein in A431, HeLa, and HL60 cells, normal tonsil, normal colon, and breast carcinoma tissue.
• Target Species: Human
• Content And Storage: 2°C to 8°C
• Form: Purified
• Isotype: IgG1

For Research Use Only