DSIF Mouse, Unlabeled, Clone: 17, BD

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
BDB611107	BD Biosciences 611107	150μg
BDB611106	BD Biosciences 611106	50μg

Description

In living systems, the relative amounts of any protein are controlled at many levels. For example, amounts are affected by protein degradation, regulation of the translational rates of polypeptide synthesis (translational regulation), and control of the rates of mRNA synthesis (transcriptional regulation). Transcriptional regulation involves modulation of the rate-limiting enzyme RNA polymerase. DSIF (DRB sensitivity-inducing factor) is a heterodimeric transcription elongation protein. It is composed of a large subunit of 160kDa and a small subunit of 14kDa. These large and the small subunits are homologs of the yeast gene products Stp5 and Stp4, respectively. Spt4 and 5 are transcription factors which are critically important for the activity of RNA polymerase. In conjunction with DRB, DSIF attenuates RNA polymerase II elongation steps. However, in limiting amounts of ribonucleotides, DSIF, by itself, stimulates the elongation rate of RNA polymerase II. Thus, the identification of a human regulator for transcriptional elongation will greatly enhance our understanding of this critical step in mammalian gene expression.

Host Species: Mouse
Clone: 17
Isotype: IgG1
Species Reactivity: Human
Immunogen: Human DSIF aa. 866-985
Formula Weight [Chemical]: 160kDa

Immunofluorescence, Western Blotting

Specifications

• Antigen: DSIF
• Immunogen: Human DSIF aa. 866-985
• Conjugate: Unconjugated
• Research Discipline: Cell Biology
• Host Species: Mouse
• Format: Affinity Purified
• Monoclonal or Polyclonal: Monoclonal
• Storage Requirements: Store undiluted at -20°C.

• Isotype: IgG₁
• Formulation: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Primary or Secondary: Primary
• Applications: Western Blot
• Regulatory Status: RUO
• Clone: 17
• Concentration: 250μg/mL

For Research Use Only.