E2A Mouse anti-Human, Unlabeled, Clone: G127-32, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 554077

Catalog No. BDB554077

Description

B lymphoid cells go through many intermediate steps before becoming plasma cells which produce and secrete immunoglobulins (Ig). The earliest stage of B-cell differentiation is represented by pro-B lymphocytes that contain both the Ig heavy- and light-chain genes in the transcriptionally inactive germ line configuration. As pro-B lymphocytes differentiate into pre-B cells, Ig heavy-chain genes rearrange and are transcribed and translated. However, the Ig light-chain genes are not transcribed before the pre-B cells differentiate into mature Blymphocytes. The developmental regulation of the Ig gene expression is dependent on various sequences in the Ig enhancer region. One class of such regulatory sequence elements comprises the E-boxes which share the NNCANNTGNN consensus sequence. The E2 boxes are particularly interesting because they are present in muscle and pancreas-specific enhancers. A family of proteins binds to the E2 box. These proteins share a common amino acid sequence motif that is proposed to form two amphipathic helices interrupted by a loop, designated the helix-loop-helix (HLH) motif. The HLH motif mediates homo- as well as heterodimerization with other HLH proteins. Most HLH proteins possess a basic region located at the N terminal of the HLH region which is responsible for DNA binding. Two E2 box binding proteins have been described (E12 and E47) that arise as alternatively spliced form of the E2A gene. E12 and E47 are identical except in the HLH region. These two proteins have been directly implicated in the regulation of B-cell, muscle and pancreas-specific gene expression. Futhermore, studies have shown that relative levels of E47 are important for regulating the lineage of T cells in the thymus. For example, mice deficient in E47 demonstrate a change in the ratio of CD4/CD8 cells as well as a greater percentage of cells that stain for the mature CD4 marker, implicating E47 in selection of T cells. Clone G127-32 recognizes human E47. It cross-reacts with mouse E47. G127-32 does not recognize E12, the alternately spliced product of the E2A gene or E2-2, a closely reltated protein. Recombinant E47 was used as immunogen.

Host Species: Mouse
Clone: G127-32
Isotype: IgG1
Species Reactivity: Human
Immunogen: Recombinant Human E47
Formula Weight [Chemical]: 75kDa

Immunoprecipitation, Western Blotting

Specifications

• Antigen: E2A
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Description: E47(E2A), Immunoglobulin E2-Box Binding Protein
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C

• Applications: Western Blot
• Clone: G127-32
• Conjugate: Unconjugated
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Recombinant Human E47
• Quantity: 0.1 mg
• Research Discipline: Cell Biology
• Target Species: Human, Mouse
• Isotype: IgG1 κ

For Research Use Only.