eIF-4E Mouse, Unlabeled, Clone: 87, BD
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 610269
The eukaryotic translation initiation factor 4E (eIF-4E) is a 25kDa phosphoprotein that specifically binds to the 7-methylguanosine-containing cap of mRNA. eIF-4E is the rate-limiting component for the initiation of cap-dependent translation by the eIF-4E translation initiation complex. This complex promotes the unwinding of secondary structure at the 5 ft. untranslated region of mRNA, which is necessary to expose and locate the AUG-initiation codon. Phosphorylation of eIF-4E on Ser-209 occurs after serum treatment in CHO cells, and may regulate its function. Overexpression of eIF-4E can lead to increased cell proliferation, transformation, and tumorigenesis in nude mice. The overexpression of a Ala-53 variant of eIF-4E cannot evoke these changes, suggesting that Ser-53 on eIF-4E participates in the transfer of mRNA to the 48S initiation complexes. In cooperation with nuclear oncogenes such as c-myc or E1A, eIF-4E transforms primary cells. Other studies have demonstrated that overexpression of eIF-4E causes activation of Ras and leads to a transformed phenotype. Subsequent overexpression of GAP then causes reversion of this phenotype. The mechanism by which eIF-4E plays a role in transformation is not clear, but it is postulated that high levels of eIF-4E may lead to the translation of mRNAs that are normally translationally repressed.
Host Species: Mouse
Species Reactivity [for Features Main]: Human
Immunogen: Rabbit eIF-4E aa. 1-217
|Aqueous buffered solution containing BSA|
|Canine, Chicken, Frog, Human, Murine, Rat|
|Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.|
|Store undiluted at -20°C.|
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