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eNOS Mouse, PE, Clone: 3/eNOS/NOS Type III, BD

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Catalog No. BDB560103 Supplier BD Biosciences Supplier No. 560103
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Description

Mouse Monoclonal Antibody

Nitric oxide synthase (NOS), a cell-type specific enzyme, catalyzes the synthesis of nitric oxide (NO). NO is a short-lived radical that transmits signals involved in vasorelaxation, neurotransmission, and cytotoxicity. In neurons and endothelial cells, constitutive NOS (cNOS) is activated by agonists that increase intracellular Ca2+ levels and enhance calmodulin binding. Neuronal NOS (nNOS) and endothelial NOS (eNOS) have recognition sites for NADPH, FAD, FMN, and calmodulin and both are regulated in a similar manner. The human forms exhibit 52% amino acid identity. However, they are distinct gene products of about 155 kDa (nNOS) and 140 kDa (eNOS). The eNOS gene was cloned from human vascular endothelium as well as from bovine aortic endothelial cells (BAEC). eNOS protein has a unique N-myristylation consensus sequence that may explain its membrane localization.

The 33/eNOS monoclonal antibody recognizes eNOS, regardless of phosphorylation status.

The specificity of this antibody conjugate for flow cytometric analysis was validated by confirming that RNA-mediated interference (RNAi) of the specific protein reduced the staining of the cells (see figure). Furthermore, the capacity of the RNAi to down-regulate the expression of the relevant protein was confirmed by western blot analysis.

TRUSTED_SUSTAINABILITY

Specifications

Antigen eNOS
Applications Flow Cytometry
Classification Monoclonal
Clone 3/eNOS/NOS Type III
Conjugate PE
Description NOS type III, NOS3, EC-NOS, NOS III
Formulation Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Host Species Mouse
Immunogen Human eNOS aa. 1025-1203
Purification Method Affinity Purified
Quantity 50 Tests
Regulatory Status RUO
Primary or Secondary Primary
Target Species Human, Mouse
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG1 κ
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