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ERK1/2 (pT202/pY204) Mouse, PE, Clone: 20A, BD

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 612566

Catalog No. BDB612566


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Description

Description

The members of the Mitogen-Activated Protein Kinase (MAPK) family are components of a key signal transduction cascade that links events at the cell surface to responses in the nucleus. The signaling cascade is found in species as varied as yeast and humans, with many of the proteins being well conserved. In mammals the most widely studied members of the cascade are the Extracellular signal-Regulated Kinases, ERK1 (p44 MAPK) and ERK2 (p42 MAPK). ERK1 and ERK2 share 85% homology and are activated by extracellular signals such as growth factors, hormones, and phorbol esters. Activation occurs through a series of phosphorylations by kinases activating other kinases and eventually leading to phosphorylation of the ERKs. Growth factor stimulation leads to activation of Ras and Raf, leading to phosphorylation of MEK1 (MAPK/ERK kinase) which, in turn, activates the ERKs via dual phosphorylation. Once activated, the ERKs phosphorylate other cytoplasmic signalling molecules, cell-surface receptors, microtubule-associated proteins, and transcription factors in the nucleus. Thus, the active ERK has myriad downstream effectors that implicate it in the control of cell proliferation and differentiation, as well as regulation of the cytoskeleton. Furthermore, studies have shown that elevated ERK activity is associated with some cancers. The 20A monoclonal antibody recognizes the phosphorylated threonine 202 and tyrosine 204 (pT202/pY204) of human ERK1 and pT184/pY186 of human ERK2. The orthologous phosphorylation sites in murine ERK1 and ERK2 are T203/Y205 and T183/Y185.

Intracellular Staining

Specifications

Specifications

ERK1/2 (pT202/pY204)
20A
PE
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Murine
IgG1
RUO
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
Flow Cytometry
250μg/mL
p44/42 MAPK; Extracellular signal-Regulated Kinase 1/2 (pT202/Y204)
Affinity Purified
Phosphorylated Rat ERK1 (T202/Y204) Peptide
50 Tests
Cell Biology
Primary
Human, Murine, Rat
Documents
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