Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Estrogen Receptor beta Rabbit anti-Human, Mouse, Rat, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Manufacturer:  Invitrogen PA1313

Catalog No. PA1313


Add to cart

Description

Description

PA1-313 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects an ~55 kDa protein representing ER beta from rat brain homogenate. Immunohistochemical staining of ER beta in rat prostate with PA1-313 results in nuclear staining, whereas nuclear staining may not be observed in human tissue. The PA1-313 immunogen is a synthetic peptide corresponding to residues C(459) S P A E D S K S K E G S Q N P Q S Q(477) of human ER beta. PA1-313 immunizing peptide (Cat. No. PEP-029) is available for use in neutralization and control experiments. Recombit ER beta expressed in baculovirus system (Cat. No. RP-311 and RP-312) can be purchased for control experiments in Western blot and gel shift assays.

Estrogen Receptor beta (ER beta, NR3A2) protein is approximately 55kD, though multiple isoforms ranging from 36-59kD have been described. Human ER beta was initially cloned and characterized from testis. Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Estrogen receptor beta (ER beta) binds estrogens with an affinity similar to that of estrogen receptor alpha. Some ER beta isoforms domitly inhibit the activity of estrogen receptor alpha in reproductive tissues. ER beta is found widely in many tissues throughout the body and can act as a potent tumor suppressor, playing a crucial role in many cancer types such as prostate cancer, autism spectrum disorder, medullary thyroid carcinoma, De Quervain Disease and risk of cardiovascular disease.
Specifications

Specifications

Estrogen Receptor beta
Polyclonal
Unconjugated
ESR2
Liquid
ESR2
Synthetic peptide corresponding to residues C(459) S P A E D S K S K E G S Q N P Q S Q(477) of human ER beta.
Antigen affinity chromatography
RUO
Antibody
Polyclonal
Human, Mouse, Rat
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
1 mg/mL
PBS with 1mg/mL BSA and 0.05% sodium azide
O08537, Q62986, Q92731
ER Beta, ER, ESR2, ESR-Beta
Rabbit
IgG
50 μg
-20° C, Avoid Freeze/Thaw Cycles
Primary
13983, 2100, 25149
Documents
Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit