FAK (pY397) (Focal Adhesion Kinase (pY397)) Mouse anti-Human, Unlabeled, Clone: 18, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 611807

Catalog No. BDB611807

Description

Focal Adhesion Kinase (FAK) is a cytoplasmic tyrosine kinase that colocalizes with integrins in focal adhesions. This cellular localization is directed by a 125 amino acid sequence at the C-terminus called “Focal Adhesion Targeting” sequence (FAT). The binding of extracellular matrix ligands to integrins triggers autophosphorylation at Tyr-397, and activation of FAK through phosphorylation of Tyr residues (Tyr-576 and Tyr-577) in the kinase domain activation loop. For example, cell adhesion to a fibronectin substratum involves concurrent activation of Src and phosphorylation of the FAK activation loop. In addition, phosphorylation of other Tyr residues (Tyr-925 and Tyr-861) creates binding sites for SH2 domains of intracellular signaling molecules such as Src, PI3 kinase, and Grb2. FAK's ability to bind numerous structural and signaling proteins via a variety of interactions is important for FAK activation level and for FAK interaction with a variety of substrates localized to sites of cell adhesion. Thus, FAK activity is regulated by a complex set of phosphorylation sites, and this phospho-regulation could be important for cell motility, cell growth, cytoskeletal organization and adhesion-dependent cell survival.

Host Species: Mouse
Clone: 18
Isotype: IgG1
Species Reactivity [for Features Main]: Human
Immunogen: Human FAK (pY397)

Bioimaging, Immunofluorescence, Western Blotting

Specifications

• Antigen: FAK (pY397) (Focal Adhesion Kinase (pY397))
• Classification: Monoclonal
• Concentration: 250μg/mL
• Description: Focal Adhesion Kinase (pY397)
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at -20°C.

• Applications: Western Blot
• Clone: 18
• Conjugate: Unconjugated
• Formulation: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Immunogen: Human FAK (pY397)
• Quantity: 150 μg
• Research Discipline: Cell Biology
• Target Species: Human
• Isotype: IgG1

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only.