FKBP-12 Mouse, Unlabeled, Clone: 2C1-87, BD

Catalog No.	Quantity
BDB554091	0.1 mg

Catalog No. BDB554091 Supplier BD Biosciences Supplier No. 554091

Description

Mouse Monoclonal Antibody

FKBP-12 (for FK506 binding protein-12kDa) is a ubiquitously expressed protein involved in T-cell signaling that binds to FK506. FK506 belongs to a class of proteins that have peptidyl-prolyl cis-trans isomerase (PPIase) activity. This class of proteins is thought to be involved in folding proteins and peptides into their native conformations. It is thought that peptidyl-prolyl isomerization may play a role in regulating components of the T-cell signal transduction pathway such as transcription factors, protein kinase activation, and ion channel function.
FK506, like cyclosporin, is an agent that inhibits T- and B-cell early activation processes that are key to an effective immune response. In T cells these agents disrupt a step in the signal transduction pathway from the T-cell receptor to cytokine genes involved in coordinating the immune response. In particular, they seem to disrupt pathways that induce a measurable rise in Ca2+. NFAT, a transcription factor essential for early T-cell activation, appears to be a specific target of FK506 and cyclosporin because transcription directed by NF-AT is specifically blocked in T cells treated with these agents. NFAT is formed when a signal from the T-cell antigen receptor induces a pre-existing cytoplasmic subunit to translocate to the nucleus and combine with a newly synthesized nuclear subunit of NFAT. When FK506 binds to FKBP-12, the complex blocks translocation of the cytoplasmic subunit of NF-AT into the nucleus by inhibiting a Ca2+-dependent phosphatase. Since the cytoplasmic subunit is not translocated into the nuclear, the formation of NFAT and its subsequent transcriptional activity is blocked. The reduced molecular weight of FKBP-12 is 12kDa. The 2C1-87 antibody recognizes FKBP-12. Specifically it has been shown to recognize human, mouse, and rat FKBP-12. Recombinant FKBP-12 was used as immunogen. The antibody was originally characterized by biochemical, immunohistochemical, and functional assays.

Host Species: Mouse
Clone: 2C1-87
Isotype: IgG1
Species Reactivity: Human
Immunogen: Recombinant FKBP-12
Formula Weight [Chemical]: 12kDa

Immunohistochemistry (Paraffin), Western Blotting

Specifications

• Antigen: FKBP-12
• Applications: Western Blot
• Classification: Monoclonal
• Clone: 2C1-87
• Concentration: 0.5mg/mL
• Conjugate: Unconjugated
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Mouse
• Immunogen: Recombinant FKBP-12
• Purification Method: Affinity Purified
• Quantity: 0.1 mg
• Regulatory Status: RUO
• Research Discipline: Cell Biology
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Content And Storage: Store undiluted at 4°C
• Isotype: IgG1

For Research Use Only.