Foxp3 Rat anti-Mouse, Alexa Fluor 488, Clone: MF23, BD

Rat Monoclonal Antibody

Supplier:  BD Biosciences 560403

Catalog No. BDB560403

Description

Foxp3 is a 50-55kDa protein also known as Forkhead box P3, JM2, or IPEX. It is a member of the forkhead or winged helix family of transcription factors and is specifically expressed by T regulatory (Treg) cells. Foxp3 has been reported to be a key regulatory protein for Treg cell development and function. Ectopic expression of Foxp3 in conventional T cells is sufficient to induce suppressive activity, repress the production of cytokines such as IL2 and IFN-γ, and upregulate Treg cell-associated molecules such as CD25, CTLA4 and GITR. It has been found that the mutation of Foxp3 is responsible for “scurfy” mice. When overexpressed, Foxp3 leads to poor T cell proliferation and activation.
Monoclonal antibody MF23 reacts with mouse Foxp3. Immunoblotting with MF23 antibody has confirmed it recognizes an epitope between 1-87 amino acids in the N-terminal domain.

Host Species: Rat
Clone: MF23
Isotype: IgG2b
Species Reactivity: Mouse
Immunogen: Mouse Foxp3 Recombinant Protein

Intracellular Staining

Specifications

• Antigen: Foxp3
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Description: Forkhead box p3; Forkhead box protein p3; JM2; Scurfin; Scurfy; Sf
• Host Species: Rat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: MF23
• Conjugate: Alexa Fluor 488
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Mouse Foxp3 Recombinant Protein
• Quantity: 0.1 mg
• Research Discipline: Immunology
• Target Species: Mouse
• Isotype: IgG2b

For Research Use Only.