FoxP3 Mouse anti-Human, PE-CF594, Clone: 259D/C7, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 562421

Catalog No. BDB562421

Description

The 259D/C7 antibody monoclonal antibody specifically binds to the human FoxP3 transcription factor, a member of the forkhead or winged helix family of transcription factors. The expression of FoxP3, also known as Scurfin, IPEX and JM2, has been found to be associated with CD4+ regulatory T cells and represents a specific marker for these cells. Flow cytometric analysis has shown that FoxP3 is expressed by the majority of CD4+CD25+high T cells in peripheral blood while less than half of CD4+CD25int cell population are FoxP3 positive. Approximately 5-10% of peripheral CD4+ cells are CD4+CD25+ T regulatory cells. T regulatory cells are thought to play a critical role in the control of T cell mediated autoimmunity by suppressing the proliferation and cytokine production of other T cells. To support this hypothesis, it has been found that FOXP3 is mutated in scurfy (sf) mice. The 259D/C7 antibody reacts with all currently identified isoforms of human FoxP3 and is cross-reactive with Cynomolgus, Rhesus and Baboon. This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red™. PE-CF594 excites and emits at similar wavelengths to PE-Texas Red™ yet exhibits improved brightness and spectral characteristics. Due to PE having maximal absorption peaks at 496nm and 564nm, PE-CF594 can be excited by the blue (488nm), green (532nm) and yellow-green (561nm) lasers and can be detected with the same filter set as PE-Texas Red™ (eg 610/20nm filter).

Host Species: Mouse
Clone: 259D/C7
Isotype: IgG1
Species Reactivity: Human
Immunogen: FoxP3 recombinant protein

Intracellular Staining

Specifications

• Antigen: FoxP3
• Classification: Monoclonal
• Conjugate: PE-CF594
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Immunogen: FoxP3 recombinant protein
• Quantity: 50 Tests
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Applications: Flow Cytometry
• Clone: 259D/C7
• Description: Forkhead box P3; IPEX; JM2; PIDX: Scurfin; XPID
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Human
• Isotype: IgG1

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov