FoxP3 Mouse anti-Human, V450, Clone: 236A/E7, BD

Catalog No.	Quantity
BDB561182	100 Tests

Catalog No. BDB561182 Supplier BD Biosciences Supplier No. 561182

Description

Mouse Monoclonal Antibody

The 236A/E7 antibody reacts with the human FoxP3 (Forkhead box protein P3) transcription factor, a member of the forkhead or winged helix
family of transcription factors. The expression of FoxP3, also known as Scurfin, IPEX and JM2, has been found to be associated with CD4+
CD25+ regulatory T cells and represents a specific marker for these cells. Flow cytometric analysis has shown that FoxP3 is expressed by the
majority of CD4+ CD25+ (high) T cells in peripheral blood while less than half of CD4+ CD25+ (intermediate) cell population are FoxP3
positive. Approximately 5-10% of peripheral CD4+ cells are CD4+ CD25+ T regulatory cells. T regulatory cells are thought to play a critical
role in the control of T cell mediated autoimmunity by suppressing the proliferation and cytokine production of other T cells. To support this
hypothesis, it has been found that Foxp3 is mutated in scurfy (sf) mice. Cumulative evidence suggests that the 236A/E7 antibody recognizes
epitopes from both isoforms of Exon 2 alternatively-spliced variants and is located in the region between Exon 2 and the Zn/LZ domains (aa
105-235). The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is
available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406 nm and has an Em Max at 450 nm. Conjugates with
BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.

Host Species: Mouse
Clone: 236A/E7
Isotype: IgG1κ
Species Reactivity: Human
Immunogen: Human full-length FoxP3 Recombinant Protein

Flow Cytometry, Intracellular Staining

Specifications

• Antigen: FoxP3
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: 236A/E7
• Conjugate: Violet Fluor 450 (V450)
• Description: Forkhead box protein P3; Scurfin; AIID; IPEX; JM2; DIETER; PIDX; XPID
• Formulation: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
• Host Species: Mouse
• Immunogen: Human full-length FoxP3 Recombinant Protein
• Purification Method: Affinity Purified
• Quantity: 100 Tests
• Regulatory Status: RUO
• Research Discipline: Immunology
• Primary or Secondary: Primary
• Target Species: Human
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG1 κ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov