Foxp3 Rat anti-Mouse, V450, Clone: MF23, BD

Rat Monoclonal Antibody

Supplier:  BD Biosciences 561293

Catalog No. BDB561293

Description

The MF23 monoclonal antibody specifically binds to mouse Foxp3. Foxp3 is a 50-55 kDa protein also known as Forkhead box p3, JM2, or IPEX. It is a member of the forkhead or winged helix family of transcription factors and is specifically expressed by T regulatory (Treg) cells. Foxp3 has been reported to be a key regulatory protein for Treg cell development and function. Ectopic expression of Foxp3 in conventional T cells is sufficient to induce suppressive activity, repress the production of cytokines such as IL2 and IFN- γ, and upregulate Treg cell-associated molecules such as CD25, CTLA4 and GITR. It has been found that the mutation of Foxp3 is responsible for “scurfy” mice. When overexpressed, Foxp3 leads to poor T cell proliferation and activation. Immunoblotting with MF23 antibody has confirmed it recognizes an epitope between 1-87 amino acids in the N-terminal domain.

The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406nm and has an Em Max at 450 nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.

Specifications

• Antigen: Foxp3
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Description: Forkhead box p3; Forkhead box protein p3; JM2; Scurfin; Scurfy; Sf
• Host Species: Rat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Isotype: IgG2b

• Applications: Flow Cytometry
• Clone: MF23
• Conjugate: Violet Fluor 450 (V450)
• Formulation: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
• Immunogen: Mouse Foxp3 Recombinant Protein
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.