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  6. anti-Gαo G-Protein Ab-2, Clone: 2A.3, Epredia™ LabVision

anti-Gαo G-Protein Ab-2, Clone: 2A.3, Epredia™ LabVision

Catalog No. MS242P1ABX Shop All Epredia Products
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Quantity:
100 μL
200 μL
500 μL
1 mL
Concentration:
1mg/mL
200μg/mL
250μg/100μL
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Catalog No. Quantity Concentration
MS242P1ABX 100 μL 1mg/mL
MS242P0 100 μL 200μg/mL
MS242P1 500 μL 200μg/mL
MS242P 1 mL 200μg/mL
MS242PCL 100 μL 250μg/100μL
MS242PABX 200 μL 1mg/mL
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Catalog No. MS242P1ABX Supplier Epredia Supplier No. MS242P1ABX

Description

Mouse Monoclonal Antibody

GTP-binding proteins (G-proteins) play a critical role in signal transduction by coupling receptors to effectors. The intracellular consequences of G-protein activation include second messenger generation, protein phosphorylation, ion channel activation, gene induction, cell growth, and differentiation. Go belongs to the pertussis toxin sensitive G-proteins. It consists of two subtypes of α-subunits (αo1 and αo2) derived from alternative splicing of a single gene. αo1 differs from αo2 within the C-terminal third of the deduced amino acid sequence.

Host Species: Mouse
Clone: 2A.3
Isotype: IgG1
Cross Reactivity: Human, Cow, Rat, Mouse, and Guinea pig. Others-not known
Epitope: Not determined
Immunogen: Partially purified bovine brain Gαo protein
Molecular Weight: 39-42kDa
Positive Control: IMR-5 cells. Brain
Cellular Localization: Cell membrane

Ab-2 is highly specific to Galphao and shows no cross-reaction with transducin, Gαi1, Gαi2, Gαi3, or Gαs. It reacts equally with the C-terminal splice variants of Gαo (mouse GαoA and GαoB, equivalent to Gαo1 and Gαo2 in other species).

Western Blotting

Abbreviations: AP=Affinity Purification, ChIP=Chromatin IP, CM=Confocal Microscopy, DB=Dot Blot, EIA=ELISA, EM=Electron Microscopy, FC=Flow Cytometry, GS=Gel Supershift, IC=Immunocytochemistry, IF=Immunofluorescence, IH=Immunohistology/Immunohistochemistry, IP=Immunoprecipitation, IPK=IP Kinase Assay, KA=Kinase Assay, NEUT=Neutralization, PIA=Peptide Inhibition Assay, RIA=Radioimmunossay, TCA=T Cell Activation, WB=Western Blotting, Species: Am=Amphibian, Av=Avian, B=Bovine, Ca=Cat ,Ck=Chicken, D=Dog, Dr=Deer, Ds=Drosophila, El=Elk, Fg=Frog, Fs=Fish, Ft=Ferret, G=Goat, Gp=Guinea Pig, H=Horse, Hm=Hamster, Hu=Human, K=Kangaroo, Ms=Mouse, Nhp=Non-Human Primate, O=Ovine, P=Porcine, Rb=Rabbit, Rp=Reptile, Rt=Rat, Ys=Yeast, Xn=Xenopus, (wk)=cross reacts weakly

Specifications

Antigen Gαo G-Protein Ab-2
Classification Monoclonal
Concentration 1mg/mL
Conjugate Unconjugated
Host Species Mouse
Immunogen Partially purified bovine brain Gαo protein
Quantity 100 μL
Primary or Secondary Primary
Target Species Bovine, Guinea Pig, Human, Mouse, Rat
Form Purified
Isotype IgG1
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