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Gαt Mouse, Unlabeled, Clone: 3, BD

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 610589

Catalog No. BDB610589

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The GTP binding regulatory proteins (G proteins) consist of three subunits: α, β, and γ. These heterotrimeric proteins function at membranes to relay signals from cell surface receptors to intracellular effectors. The α subunit is unique for each G protein and contains the site of GTP binding and hydrolysis, as well as sites for receptor and effector interactions. The βγ subunit complex interacts directly with receptors and the α subunit. The Gα protein transducin (Gαt) contains 350 amino acids and has been extensively studied as a model for G protein function. Gαt requires GTP in order to bind to its effectors. In the process of effector-Gαt binding, GTP is hydrolyzed and the βγ subunits are displaced. The free Gαt-GDP then reassociates with the βγ subunits and re-loads GTP to repeat the cycle.

Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting



Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
Store undiluted at -20°C.
Western Blot
Gα protein transducin
Affinity Purified
Bovine Gαt aa. 282-300
Cell Biology
Chicken, Human, Murine, Rat
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