Gαt Mouse, Unlabeled, Clone: 3, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 610589

Catalog No. BDB610589

Description

The GTP binding regulatory proteins (G proteins) consist of three subunits: α, β, and γ. These heterotrimeric proteins function at membranes to relay signals from cell surface receptors to intracellular effectors. The α subunit is unique for each G protein and contains the site of GTP binding and hydrolysis, as well as sites for receptor and effector interactions. The βγ subunit complex interacts directly with receptors and the α subunit. The Gα protein transducin (Gαt) contains 350 amino acids and has been extensively studied as a model for G protein function. Gαt requires GTP in order to bind to its effectors. In the process of effector-Gαt binding, GTP is hydrolyzed and the βγ subunits are displaced. The free Gαt-GDP then reassociates with the βγ subunits and re-loads GTP to repeat the cycle.

Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting

Specifications

• Antigen: Gαt
• Classification: Monoclonal
• Concentration: 250μg/mL
• Description: Gα protein transducin
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at -20°C.

• Applications: Western Blot
• Clone: 3
• Conjugate: Unconjugated
• Formulation: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Immunogen: Bovine Gαt aa. 282-300
• Quantity: 150 μg
• Research Discipline: Cell Biology
• Target Species: Chicken, Human, Mouse, Rat
• Isotype: IgG1