MilliporeSigma™ anti-GD3, 9-O-acetyl Clone: UM4D4,

Catalog No.	Quantity	Conjugate
MABF978MI	100 μL	Unconjugated

Catalog No. MABF978MI Supplier MilliporeSigma™ Supplier No. MABF978

Description

Mouse Monoclonal Antibody

Specifically detects GD3, 9-O-acetyl Clone: UM4D4 in Human samples, and it is validated for Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry

Gangliosides are sialic acid-containing oligoglycosylceramides. The German scientist Ernst Klenk used ganglioside in 1942 to refer to lipids isolated from ganglions. Gangliosides are grouped based on the number of sialic acid residues per molecule, GM gangliosides contain one sialic acid residue, GD gangliosides contain two, GT contain three, GQ contain four sialic acid residues. Sialyltransferase-catalyzed α2-3 sialylation of the galactose of the trisaccharide Galβ1-4Glcβ1-1'Cer produces GM3, which can be further sialylated to produce GD3 with two sialic acid on the galactose. A de-N-acetylated form of ganglioside GD3 has been detected in human melanoma tumors. In addition, O-acetylation or lactonization of the sialic acid residue adds to the potential complexity. Gangliosides containing O-acetylated sialic acids, such as 9-OAc-GD3, occur in certain tumors and may protect cancer cells from apoptosis. GD3 is also known as CD60a. 9-O-acetylated GD3 is known as CDw60 or CD60b, while 7-O-acetylated GD3 is known as CD60c.

Specifications

• Antigen: GD3, 9-O-acetyl
• Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
• Classification: Monoclonal
• Clone: UM4D4
• Conjugate: Unconjugated
• Formulation: Purified mouse monoclonal IgMκ antibody in PBS without preservatives.
• Host Species: Mouse
• Immunogen: Human rheumatoid synovial T cell line ST-1.
• Purification Method: Purified by ion-exchange chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Inflammation, Immunology
• Primary or Secondary: Primary
• Test Specificity: Clone UM4D4 detects 9-O-acetylated ganglioside GD3. GD3 is also known as CD60a. 9-O-acetylated GD3 is known as CDw60 or CD60b, while 7-O-acetylated GD3 is known as CD60c.
• Target Species: Human
• Content And Storage: -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
• Form: Purified
• Isotype: IgM κ

For Research Use Only