Learn More
Description
GFAP (Glial Fibrillary Acid Protein) is the major protein of glial filaments in differentiated astrocytes. BD Pharmingen offers a panel of monoclonal antibodies (4A11, 1B4, 2E1) that specifically recognize GFAP. They do not cross-react with other intermediate filaments such as vimentin, neurofilament proteins, desmin, keratin, neurotubules or microfilaments. Bovine spinal cord homogenate was used as immunogen. This antibody has broad species reactivity, recognizing GFAP in brain homogenates from human, mouse, rat, cow, sheep, dog, pig, rabbit, guinea pig and chicken. 1B4 is particularly useful for identifying GFAP in immunohistochemistry of frozen and formalin-fixed, paraffin-embedded brain tissue sections. Additional applications include western blot analysis and indirect immunofluorescence of tissue-cultured cells. The antibody is routinely tested by immunohistochemical staining of formalin-fixed, paraffin-embedded brain tissue samples, which were subjected to pretreatment with citrate or tissue unmasking fluid (see figure). Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Host Species: Mouse
Clone: 1B4
Isotype: IgG2b, κ
Species Reactivity: Rat
Immunogen: Cow spinal cord homogenate
Immunofluorescence, Immunohistochemistry, Western Blotting
Specifications
Specifications
| Antigen | GFAP |
| Applications | Immunohistochemistry |
| Classification | Monoclonal |
| Clone | 1B4 |
| Concentration | 0.5mg/mL |
| Conjugate | Unconjugated |
| Formulation | Aqueous buffered solution containing ≤0.09% sodium azide. |
| Host Species | Mouse |
| Immunogen | Cow spinal cord homogenate |
| Purification Method | Affinity Purified |
| Show More |
Safety and Handling
For Research Use Only.
By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.