GFAP Mouse, Unlabeled, Clone: 1B4, BD

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 556328

Catalog No. BDB556328

Description

GFAP (Glial Fibrillary Acid Protein) is the major protein of glial filaments in differentiated astrocytes. BD Pharmingen offers a panel of monoclonal antibodies (4A11, 1B4, 2E1) that specifically recognize GFAP. They do not cross-react with other intermediate filaments such as vimentin, neurofilament proteins, desmin, keratin, neurotubules or microfilaments. Bovine spinal cord homogenate was used as immunogen. This antibody has broad species reactivity, recognizing GFAP in brain homogenates from human, mouse, rat, cow, sheep, dog, pig, rabbit, guinea pig and chicken. 1B4 is particularly useful for identifying GFAP in immunohistochemistry of frozen and formalin-fixed, paraffin-embedded brain tissue sections. Additional applications include western blot analysis and indirect immunofluorescence of tissue-cultured cells. The antibody is routinely tested by immunohistochemical staining of formalin-fixed, paraffin-embedded brain tissue samples, which were subjected to pretreatment with citrate or tissue unmasking fluid (see figure). Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Host Species: Mouse
Clone: 1B4
Isotype: IgG2b, κ
Species Reactivity: Rat
Immunogen: Cow spinal cord homogenate

Immunofluorescence, Immunohistochemistry, Western Blotting

Specifications

• Antigen: GFAP
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Cow spinal cord homogenate
• Quantity: 0.5 mg
• Research Discipline: Cell Biology
• Target Species: Bovine, Canine, Chicken, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat, Sheep
• Isotype: IgG2b

• Applications: Immunohistochemistry
• Clone: 1B4
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C

For Research Use Only.